Expertise

The scientific interests of the founding members of the CRIFF are in the fields of pharmacology, human genetics, statistical models, clinical pathology and medicinal chemistry. The synergic collaborations therefore allow for a comprehensive approach to pharmacogenetic problems: from the design of the protocols to the possible pharmacological implications for the patient. Each pharmacogenetic protocol or project is also implemented by clinical expertise. It is envisaged that the clinical component of the Center will grow in the near future.

The current areas of clinical interest of the members of the CRIFF are: neurology, psychiatry, hematology, oncology, endocrinology, nephrology and pneumology.

Briefly, the expertise that the members bring to the Center are as follows:

Pharmacology: pharmacogenetic genotyping via restriction enzyme digestion, genotyping via real-time PCR, various cellular models to understand the role of polymorphisms on pharmacodynamic targets. Analysis of telomerase expression, analysis of cultured patient monocytes for abherrant expression of proteins.

Medicinal chemistry: determination of plasma levels of drugs, analysis of drug metabolism in animal models and in vitro, including possible drug-drug interactions.

Statistics: simple and complex mathematical and statistical models, algorithms for microarray analysis.

Equipments

he members of the CRIFF belong to University departments (Department of Pharmaceutical Sciences and DiSM) that have equipment that is of particular relevance to the activities of the CRIFF. For example, FACS and MS-HPLC belong to Department of Pharmaceutical Sciences. One of the main objectives of the CRIFF is also to allow shared use of equipment under the responsibility of single users. To this end, the CRIFF has access to a number of thermocyclers, real-time PCRs, UV and fluorimetric HPLCs.